Quercetin prevents primordial follicle loss via suppression of PI3K/Akt/Foxo3a pathway activation in cyclophosphamide-treated mice

Abstract Background Chemotherapy improves the survival rates of patients with various cancers but often causes some adverse effects, including ovarian damage, characterised by a decrease in primordial follicle stockpiles. Recent studies have revealed that chemotherapy may stimulate PI3K signalling pathway, thereby resulting accelerated activation and decreased reserve. Quercetin is an inhibitor pathway; however, its protective effects against chemotherapy-induced loss mice not been established. In this study, quercetin mouse model cyclophosphamide-induced dysfunction were investigated. Methods C57BL/6 female used for study. Paraffin sections ovaries ( n = 30 mice) stained haematoxylin eosin differential counts. Apoptosis 5 per group) was evaluated TUNEL assay. Immunohistochemical staining ki67 Foxo3a performed to evaluate follicles. The role pathway 45 assessed western blotting. Results attenuated reduction dormant Analysis PI3K/Akt/Foxo3a showed phosphorylation proteins ovaries. Furthermore, prevented apoptosis early growing follicles antral follicles, maintained anti-Müllerian hormone levels secreted these preserved quiescence pool, as determined intranuclear staining. Conclusions attenuates preventing members maintaining level through reduced Accordingly, expected improve fertility preservation prevention endocrine-related side chemotherapy.